Is there an upper limit to the size of image files imported to Columbus?

I have been unable to successfully import TIF files > 30 MB. I am using the Import Type method 'Columbus CSV' and have had no issues importing TIF files < 30 MB. The server Job Status indicates 'Completed' for these large files but when I navigate to the images (Browse Screen), the following error appears:

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/usr/local/PerkinElmerCTG/Acapella-4.1.2/AcapellaResources/AcapellaColumbusWebapp/ProcLib/columbus/navigator.proc(260) [Columbus::nav_DisplayImage]: 
/usr/local/PerkinElmerCTG/Acapella-4.1.2/AcapellaResources/AcapellaColumbusWebapp/ProcLib/columbus/util_imageview.proc(262) [::Assert]: Assertion failed: status
status: 0

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A possible solution to my problem may require tiling/segmenting these large image files into smaller image files?

Hi,

What is the resolution of the images which are >30Mb? Columbus uses the OMERO ICE interface for import so images are limited to smaller than approx. 3000x3000.

Best,

Nick

Hi Nick,

Thanks for the response and info. My images >30Mb are approximately 10000x8000 (2 micron resolution).

Would segmenting the images be my best workaround or can you recommend any other alternative(s) for Columbus import?

Best,

Devin

Hi Devin,

Yes, you'll need to segment the images in order to get them into Columbus. Are these stitched images? If so, do you have copies of the raw tiles, with overlap?

Best,

Nick